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KMID : 0616120120430040286
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 4 p.286 ~ p.290
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Quantitative Analysis of Kirenol in Siegesbeckia glabrescens and S. pubescens by HPLC-UV
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Agung Nugroho
Lee Kyung-Tae
Park Hee-Juhn
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Abstract
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Many diterpenoids from Siegesbeckia species (Compositae) and their anti-inflammatory actions have been examined. In this research, high-performance liquid chromatography-ultraviolet spectrophotometer (HPLC-UV) method was used to compare the quantitative level of kirenol (ent-pimarane-type diterpenoid) in the aerial parts of Korean S. glabrescens and S. pubescens and the Chinese Siegesbeckiae Herba. Fingerprints of the two HPLC chromatograms of Korean S. glabrescens and S. pubescens were similar, but considerably different from Chinese Siegesbeckiae Herba. The content of kirenol in S. pubescens (16.51±0.10 mg/ml dry weight as mean±RSD) was higher than S. glabrescens (13.48±0.12 mg/g dry weight). These values were considerably higher than the Chinese Siegesbeckiae Herba (1.55±0.74 mg/g dry weight). Thin layer chromatography (TLC) analysis demonstrated the containing of kirenol in the three plant materials, but the presence of siegeskaurolic acid (entkaurane-type diterpenoid) only in the Chinese Siegesbeckiae Herba.
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KEYWORD
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Siegesbeckia glabrescens , Siegesbeckia pubescens , Siegesbeckiae Herba , HPLC-UV , Kirenol , macrophage
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